{"id":514,"date":"2019-02-22T18:18:57","date_gmt":"2019-02-22T18:18:57","guid":{"rendered":"http:\/\/www.trasferimentotecnologico.nano.cnr.it\/?page_id=514"},"modified":"2019-04-01T13:36:38","modified_gmt":"2019-04-01T13:36:38","slug":"validazione-di-modelli-in-vivo","status":"publish","type":"page","link":"https:\/\/trasferimentotecnologico.nano.cnr.it\/en\/validazione-di-modelli-in-vivo\/","title":{"rendered":"Validation of in vivo models"},"content":{"rendered":"<div data-elementor-type=\"wp-post\" data-elementor-id=\"514\" class=\"elementor elementor-514 elementor-bc-flex-widget\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-fe8ae8b elementor-section-stretched elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"fe8ae8b\" data-element_type=\"section\" data-e-type=\"section\" data-settings=\"{&quot;stretch_section&quot;:&quot;section-stretched&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-72211cc\" data-id=\"72211cc\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-53e9a48 elementor-widget elementor-widget-text-editor\" data-id=\"53e9a48\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>\u00a0<\/p><p>\u00a0<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0b2ab11 elementor-widget elementor-widget-image\" data-id=\"0b2ab11\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"525\" height=\"350\" src=\"https:\/\/trasferimentotecnologico.nano.cnr.it\/wp-content\/uploads\/2019\/02\/7-Ratto-768x512.png\" class=\"attachment-medium_large size-medium_large wp-image-666\" alt=\"\" srcset=\"https:\/\/trasferimentotecnologico.nano.cnr.it\/wp-content\/uploads\/2019\/02\/7-Ratto-768x512.png 768w, https:\/\/trasferimentotecnologico.nano.cnr.it\/wp-content\/uploads\/2019\/02\/7-Ratto-300x200.png 300w, https:\/\/trasferimentotecnologico.nano.cnr.it\/wp-content\/uploads\/2019\/02\/7-Ratto-1024x683.png 1024w, https:\/\/trasferimentotecnologico.nano.cnr.it\/wp-content\/uploads\/2019\/02\/7-Ratto.png 1665w\" sizes=\"100vw\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Dynamic imaging and simultaneous electroencephalogram (EEG) recording in the mouse cerebral cortex in response to hypercapnia. (A, B) Schematic diagram of the imaging process. Cortical neurons express a protein whose fluorescence depends on both pH and intracellular chlorine concentration. Simultaneous measurement of pH and Chlorine is obtained by combining images produced by exciting the sensor at 910 and 860 nm (see Sulis Sato et al, PNAS 2017 for details). C,D) EEG measured in mouse cortex: during hypoxia caused by breathing a 30% CO2 mixture, a strong reduction in electrophysological activity is observed to which corresponds a strong acidification of the intracellular environment (E). (F) Typical images obtained under a two-photon microscope during acquisition (20-micron bar).<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-58321c8 elementor-widget elementor-widget-text-editor\" data-id=\"58321c8\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>CONTACT:\u00a0<\/strong><br \/><em style=\"font-size: 1rem;\"><strong>Gian Michele Ratto\u00a0<\/strong><\/em><span style=\"font-size: 1rem;\">(<\/span><a style=\"background-color: #ffffff; font-size: 1rem;\" href=\"mailto:gianmichele.ratto@nano.cnr.it\">gianmichele.ratto@nano.cnr.it<\/a><span style=\"font-size: 1rem;\">)<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-974ab4c elementor-widget elementor-widget-text-editor\" data-id=\"974ab4c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"translation-block\">Areas of interest: <em>LIFE SCIENCES; BIOMEDICINE; ELECTROPHYSIOLOGY<em><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-89c4920 elementor-widget elementor-widget-text-editor\" data-id=\"89c4920\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"translation-block\"><strong>TECHNOLOGICAL ACTIVITY OFFERED<\/strong><br>\nValidation of models and treatments by way of behavioral techniques, in vivo imaging by two-photon microscopy and by electrophysiology.<\/p><p class=\"translation-block\"><strong>DESCRIPTION OF RELATED RESEARCH<\/strong><br>In vivo mouse and zebrafish models of autism spectrum disorders, epilepsy and cognitive deficits.<br>In vivo mouse models of brain tumors.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>\u00a0 \u00a0 Imaging dinamico e registrazione simultanea delle elettroencefalogramma (EEG) nella corteccia cerebrale del topo in risposta alla ipercapnia. A, B) Schema del processo di imaging. I neuroni corticali esprimono una proteina la cui fluorescenza dipende sia dal pH che dalla concentrazione di cloro intracellulare. La misura simultanea di pH e Cloro \u00e8 ottenuta combinando &hellip; <\/p>\n<p class=\"link-more\"><a href=\"https:\/\/trasferimentotecnologico.nano.cnr.it\/en\/validazione-di-modelli-in-vivo\/\" class=\"more-link\">Continue reading<span class=\"screen-reader-text\"> &#8220;Validazione di modelli in vivo&#8221;<\/span><\/a><\/p>","protected":false},"author":4,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-514","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/trasferimentotecnologico.nano.cnr.it\/en\/wp-json\/wp\/v2\/pages\/514","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/trasferimentotecnologico.nano.cnr.it\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/trasferimentotecnologico.nano.cnr.it\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/trasferimentotecnologico.nano.cnr.it\/en\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/trasferimentotecnologico.nano.cnr.it\/en\/wp-json\/wp\/v2\/comments?post=514"}],"version-history":[{"count":18,"href":"https:\/\/trasferimentotecnologico.nano.cnr.it\/en\/wp-json\/wp\/v2\/pages\/514\/revisions"}],"predecessor-version":[{"id":805,"href":"https:\/\/trasferimentotecnologico.nano.cnr.it\/en\/wp-json\/wp\/v2\/pages\/514\/revisions\/805"}],"wp:attachment":[{"href":"https:\/\/trasferimentotecnologico.nano.cnr.it\/en\/wp-json\/wp\/v2\/media?parent=514"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}